| Column | |
|---|---|
| Mobile Phase | A: 2M ammonium sulfate + B ( pH 7.0 ) , B: 25 mM sodium phosphate ( pH 7.0 ) |
| Flow Rate | 1.0 mL/min |
| Column Temperature | 25 ℃ |
| Detector | UV 280 nm |
| Injection volume | 20 µL |
| Sample | |
| Pressure | |
| Time (min) | |
| B (%) |
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