| Column | |
|---|---|
| Mobile Phase | A: 50 mM Phosphate buffer ( pH 6.0 ), 0.15 M NaCl B: 50 mM NaH2PO4 ( pH 2.5 ), 0.1 M NaCl |
| Flow Rate | 1.0 mL/min |
| Column Temperature | 25 ℃ |
| Detector | 280 nm |
| Injection volume | 10 µL |
| Sample | Mab |
| Pressure | |
| Time (min) | |
| B (%) |
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