液相色谱柱：7.8x300 mm, 5 µm
传递相：150 mM phosphate buffer, pH 7.0
流动速度：1.0 mL/min
查重激发光谱：UV 214 nm
进样量：10 µL
样品英文：1）. Thyroglobulin, 670 kD; 2）. g-Globulin, 158 kD; 3）. BSA, 66 kD; 4）. Ovalbumin, 44 kD; 5）. Myoglobin, 17.6 kD; 6）. Ribonuclease A, 13.7 kD; 7）. B12, 1.35 kD; 8）. Uracil, 120
| 固定相 | SRT-C SEC-150 | SRT-C SEC-300 | SRT-C SEC-500 | SRT-C SEC-1000 | SRT-C SEC-2000 |
|---|---|---|---|---|---|
| 材料 | 表面键合亲水薄膜的硅胶 | 表面键合亲水薄膜的硅胶 | 表面键合亲水薄膜的硅胶 | 表面键合亲水薄膜的硅胶 | 表面键合亲水薄膜的硅胶 |
| 颗粒大小 | 5 µm | 5 µm | 5 µm | 5 µm | 5 µm |
| 孔径 (Å) | ~ 150 | ~ 300 | ~ 500 | ~ 1000 | ~ 2000 |
| 蛋白质分子量范围 | 500 - 150,000 | 5,000 – 1,250,000 | 15,000 – 5,000,000 | 50,000-7,500,000 | ＞10,000,000 |
| 水溶性聚合物分子量范围 | 500-25,000 | 1,000-100,000 | 25,000-500,000 | 5,000-1,500,000 | 50,000-＞2,500,000 |
| pH 稳定性 | 2 – 8.5 短时可耐8.5-9.5 | 2 – 8.5 短时可耐8.5-9.5 | 2 – 8.5 短时可耐8.5-9.5 | 2-8.5，短时间可耐受8.5-9.5 | 2-8.5，短时间可耐受8.5-9.5 |
| 反压 (psi for a 7.8x300 mm) | ~ 700 | ~ 700 | ~ 700 | ~ 700 | ~ 700 |
| 最大压力 (psi) | ~ 4,500 | ~ 3,500 | ~ 3,000 | ~ 3000 | ~ 3000 |
| 盐浓度范围 | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M |
| 最高使用温度 (oC) | ~ 80 | ~ 80 | ~ 80 | ~ 80 | ~ 80 |
| 流动相兼容性 | 常规水相及有机相溶剂 | 常规水相及有机相溶剂 | 常规水相及有机相溶剂 | 常规水相及有机相溶剂 | 常规水相及有机相溶剂 |
| 产品名称 | 粒度 | 孔径 | 规格 | 订货号 |
|---|---|---|---|---|
| SRT-C SEC-150 | 5 µm | 150 Å | 21.2x600 mm | 235150-21260 |
| SRT-C SEC-150 | 5 µm | 150 Å | 21.2x300 mm | 235150-21230 |
| SRT-C SEC-150 | 5 µm | 150 Å | 7.5x600 mm | 235150-7560 |
| SRT-C SEC-150 | 5 µm | 150 Å | 7.8x300 mm | 235150-7830 |
| SRT-C SEC-150 | 5 µm | 150 Å | 7.8x150 mm | 235150-7815 |
| SRT-C SEC-150 | 5 µm | 150 Å | 7.8x50 mm(保护柱) | 235150-7805 |
| SRT-C SEC-150 | 5 µm | 150 Å | 4.6x300 mm | 235150-4630 |
| SRT-C SEC-150 | 5 µm | 150 Å | 4.6x150 mm | 235150-4615 |
| SRT-C SEC-150 | 5 µm | 150 Å | 4.6x50 mm(保护柱) | 235150-4605 |
| 产品名称 | 粒度 | 孔径 | 规格 | 订货号 |
|---|---|---|---|---|
| SRT-C SEC-300 | 5 µm | 300 Å | 21.2x600 mm | 235300-21260 |
| SRT-C SEC-300 | 5 µm | 300 Å | 21.2x300 mm | 235300-21230 |
| SRT-C SEC-300 | 5 µm | 300 Å | 7.5x600 mm | 235300-7560 |
| SRT-C SEC-300 | 5 µm | 300 Å | 7.8x300 mm | 235300-7830 |
| SRT-C SEC-300 | 5 µm | 300 Å | 7.8x150 mm | 235300-7815 |
| SRT-C SEC-300 | 5 µm | 300 Å | 7.8x50 mm(保护柱) | 235300-7805 |
| SRT-C SEC-300 | 5 µm | 300 Å | 4.6x300 mm | 235300-4630 |
| SRT-C SEC-300 | 5 µm | 300 Å | 4.6x150 mm | 235300-4615 |
| SRT-C SEC-300 | 5 µm | 300 Å | 4.6x50 mm(保护柱) | 235300-4605 |
| 产品名称 | 粒度 | 孔径 | 规格 | 订货号 |
|---|---|---|---|---|
| SRT-C SEC-500 | 5 µm | 500 Å | 21.2x600 mm | 235500-21260 |
| SRT-C SEC-500 | 5 µm | 500 Å | 21.2x300 mm | 235500-21230 |
| SRT-C SEC-500 | 5 µm | 500 Å | 7.5x600 mm | 235500-7560 |
| SRT-C SEC-500 | 5 µm | 500 Å | 7.8x300 mm | 235500-7830 |
| SRT-C SEC-500 | 5 µm | 500 Å | 7.8x150 mm | 235500-7815 |
| SRT-C SEC-500 | 5 µm | 500 Å | 7.8x50 mm(保护柱) | 235500-7805 |
| SRT-C SEC-500 | 5 µm | 500 Å | 4.6x300 mm | 235500-4630 |
| SRT-C SEC-500 | 5 µm | 500 Å | 4.6x150 mm | 235500-4615 |
| SRT-C SEC-500 | 5 µm | 500 Å | 4.6x50 mm(保护柱) | 235500-4605 |
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